《植物生理学报》 2013, 49(10): 1041-1046

钙对NaCl胁迫下马铃薯脱毒苗氮代谢的影响

魏翠果, 张婷婷, 蒙美莲*, 陈有君*, 任少勇, 杨丽辉

内蒙古农业大学农学院, 呼和浩特010019

通信作者：蒙美莲；E-mail: mmeilian@126.com和cyoujun@sina.com；Tel: 0471-4303031

摘　要：

以‘克新一号’品种为试验材料, 采用组织培养方法, 研究了0、5、10、15、20 mmol·L-1 CaCl2对0、25、50、75 mmol·L-1 NaCl胁迫下马铃薯脱毒苗氮代谢的影响。结果显示, 随着NaCl胁迫浓度的增加, 马铃薯脱毒苗叶片硝态氮含量先升高后降低, 氨态氮含量持续升高, 全氮和可溶性蛋白含量以及硝酸还原酶(nitrate reductase, NR)和谷氨酰胺合成酶(glu-tamine synthetase, GS)活性持续下降。在0、25、50 mmol·L-1 NaCl胁迫下添加10 mmol·L-1 CaCl2, 以及在75 mmol·L-1 NaCl胁迫下添加15 mmol·L-1 CaCl2, 可有效缓解盐胁迫对NR和GS的抑制作用, 显著降低硝态氮和氨态氮含量, 增强氮素同化作用, 显著增加全氮和可溶性蛋白含量, 改善植株氮素营养, 减轻NaCl胁迫对植株的伤害。

关键词：马铃薯; 钙; NaCl胁迫; 氮代谢

收稿：2013-05-14   修定：2013-07-09

资助：现代农业产业技术体系建设专项资金(CARS-10-P17)

Effects of Calcium on Nitrogen Metabolism of Potato Virus-Free Seedlings under NaCl Stress

WEI Cui-Guo, ZHANG Ting-Ting, MEMG Mei-Lian*, CHEN You-Jun*, REN Shao-Yong, YANG Li-Hui

College of Agricultural, Inner Mongolia Agricultural University, Hohhot 010019, China

Corresponding author: MEMG Mei-Lian; E-mail: mmeilian@126.com和cyoujun@sina.com; Tel: 0471-4303031

Abstract:

The effects of CaCl2 of 0, 5, 10, 15 and 20 mmol·L^-1 in the culture medium on the nitrogen metabolism of potato variety of ‘Kexin No.1’ were investigated under 0, 25, 50 and 75 mmol·L^-1 NaCl stress. The results showed that the nitrate content in the leaf of virus-free potato seedling elevated with the increasing of NaCl stress concentration in the range of 0–50, and then declined. The ammonia nitrogen content in leaf increased constantly. The content of total nitrogen, soluble protein, the activity of nitrate reductase (NR) and glutamate synthase (GS) in leaf decreased continually. 10 mmol·L^-1 CaCl2 addition under the stress of 0, 25, 50 mmol·L^-1 NaCl, and 15 mmol·L^-1 CaCl2 addition under the stress of 75 mmol·L^-1 NaCl could effectively alleviate the inhibitory effect of the salt stress on NR and GS, significantly reduced the nitrate nitrogen and ammonium nitrogen contents, and significantly increased total nitrogen and soluble protein contents. These results indicated that calcium could enhance the nitrogen assimilation, improve plant nitrogen nutrition, and reduce the damage of NaCl stress on the plants.

Key words: potato; calcium; NaCl stress; nitrogen metabolism